Carbon nanotubes have turn into broadly used on account of their superior physiochemical properties, and so information of their toxicity has turn into vital. On this examine, the poisonous results of nanotubes on Caenorhabditis elegans have been investigated. Multi-walled carbon nanotubes had been characterised by transmission electron microscopy and particle measurement evaluation. Upon publicity to the nanotubes at concentrations of 100 to 500 mg/L for 3 to fifteen days, numbers and physique lengths of the nematodes weren’t altered, however physique thickness was raised and physique actions had been barely decreased. A brand new sort of toxicity-reproduction assay was carried out to see the impact of multi-walled carbon nanotubes. The circular-form aggregation of starved larvae was counted. The variety of aggregations was lowered with the rising nanotube focus by ∼27%. The concentration-dependent discount was confirmed by counting the eggs in a single worm which demonstrated the unfavourable impact of multi-walled carbon nanotubes on copy when the nematodes had been subjected to extended publicity.