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Exosomes derived from platelet-rich plasma administration in website mediate cartilage safety in subtalar osteoarthritis | Journal of Nanobiotechnology

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  • 1.

    Fernandez WG, Yard EE, Comstock RD. Epidemiology of decrease extremity accidents amongst U.S. highschool athletes. Acad Emerg Med. 2007;14(7):641–5.

    PubMed 

    Google Scholar
     

  • 2.

    Gimigliano F, Resmini G, Moretti A, Aulicino M, Gargiulo F, Gimigliano A, Liguori S, Paoletta M, Iolascon G. Epidemiology of musculoskeletal accidents in grownup athletes: a scoping overview. Medicina (Kaunas). 2021;57(10):1118. https://doi.org/10.3390/medicina57101118.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • 3.

    Herzog MM, Kerr ZY, Marshall SW, Wikstrom EA. Epidemiology of ankle sprains and power ankle instability. J Athlete Practice. 2019;54(6):603–10.


    Google Scholar
     

  • 4.

    Medina McKeon JM, Hoch MC. The ankle-joint advanced: a kinesiologic method to lateral ankle sprains. J Athlete Practice. 2019;54(6):589–602.


    Google Scholar
     

  • 5.

    Hirose Ok, Murakami G, Minowa T, Kura H, Yamashita T. Lateral ligament harm of the ankle and related articular cartilage degeneration within the talocrural joint: anatomic research utilizing aged cadavers. J Orthop Sci. 2004;9(1):37–43.

    PubMed 

    Google Scholar
     

  • 6.

    Valderrabano V, Hintermann B, Horisberger M, Fung TS. Ligamentous posttraumatic ankle osteoarthritis. Am J Sports activities Med. 2006;34(4):612–20.

    PubMed 

    Google Scholar
     

  • 7.

    Gribble PA, Bleakley CM, Caulfield BM, Docherty CL, Fourchet F, Fong DT, Hertel J, Hiller CE, Kaminski TW, McKeon PO, et al. Proof overview for the 2016 Worldwide Ankle Consortium consensus assertion on the prevalence, influence and long-term penalties of lateral ankle sprains. Br J Sports activities Med. 2016;50(24):1496–505.

    PubMed 

    Google Scholar
     

  • 8.

    Mittlmeier T, Rammelt S. Replace on subtalar joint instability. Foot Ankle Clin. 2018;23(3):397–413.

    PubMed 

    Google Scholar
     

  • 9.

    Glasson SS, Askew R, Sheppard B, Carito B, Blanchet T, Ma HL, Flannery CR, Peluso D, Kanki Ok, Yang Z, et al. Deletion of lively ADAMTS5 prevents cartilage degradation in a murine mannequin of osteoarthritis. Nature. 2005;434(7033):644–8.

    CAS 
    PubMed 

    Google Scholar
     

  • 10.

    Kamekura S, Hoshi Ok, Shimoaka T, Chung U, Chikuda H, Yamada T, Uchida M, Ogata N, Seichi A, Nakamura Ok, Kawaguchi H. Osteoarthritis improvement in novel experimental mouse fashions induced by knee joint instability. Osteoarthrit Cartil. 2005;13(7):632–41.

    CAS 

    Google Scholar
     

  • 11.

    Wikstrom EA, Hubbard-Turner T, Woods S, Guderian S, Turner MJ. Creating a mouse mannequin of power ankle instability. Med Sci Sports activities Exerc. 2015;47(4):866–72.

    PubMed 

    Google Scholar
     

  • 12.

    Hubbard-Turner T, Wikstrom EA, Guderian S, Turner MJ. Acute ankle sprain in a mouse mannequin. Med Sci Sports activities Exerc. 2013;45(8):1623–8.

    PubMed 

    Google Scholar
     

  • 13.

    Chang SH, Yasui T, Taketomi S, Matsumoto T, Kim-Kaneyama JR, Omiya T, Hosaka Y, Inui H, Omata Y, Yamagami R, et al. Comparability of mouse and human ankles and institution of mouse ankle osteoarthritis fashions by surgically-induced instability. Osteoarthrit Cartil. 2016;24(4):688–97.


    Google Scholar
     

  • 14.

    Dhillon RS, Schwarz EM, Maloney MD. Platelet-rich plasma remedy – future or development? Arthritis Res Ther. 2012;14(4):219.

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • 15.

    Kanchanatawan W, Arirachakaran A, Chaijenkij Ok, Prasathaporn N, Boonard M, Piyapittayanun P, Kongtharvonskul J. Quick-term outcomes of platelet-rich plasma injection for remedy of osteoarthritis of the knee. Knee Surg Sports activities Traumatol Arthrosc. 2016;24(5):1665–77.

    PubMed 

    Google Scholar
     

  • 16.

    Dorio M, Pereira RMR, Luz AGB, Deveza LA, de Oliveira RM, Fuller R. Efficacy of platelet-rich plasma and plasma for symptomatic remedy of knee osteoarthritis: a double-blinded placebo-controlled randomized scientific trial. BMC Musculoskelet Disord. 2021;22(1):822.

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • 17.

    Khoshbin A, Leroux T, Wasserstein D, Marks P, Theodoropoulos J, Ogilvie-Harris D, Gandhi R, Takhar Ok, Lum G, Chahal J. The efficacy of platelet-rich plasma within the remedy of symptomatic knee osteoarthritis: a scientific overview with quantitative synthesis. Arthroscopy. 2013;29(12):2037–48.

    PubMed 

    Google Scholar
     

  • 18.

    Fukawa T, Yamaguchi S, Akatsu Y, Yamamoto Y, Akagi R, Sasho T. Security and Efficacy of Intra-articular Injection of Platelet-Wealthy Plasma in Sufferers With Ankle Osteoarthritis. Foot Ankle Int. 2017;38(6):596–604.

    PubMed 

    Google Scholar
     

  • 19.

    Repetto I, Biti B, Cerruti P, Trentini R, Felli L. Conservative Remedy of Ankle Osteoarthritis: Can Platelet-Wealthy Plasma Successfully Postpone Surgical procedure? J Foot Ankle Surg. 2017;56(2):362–5.

    PubMed 

    Google Scholar
     

  • 20.

    Torreggiani E, Perut F, Roncuzzi L, Zini N, Baglio SR, Baldini N. Exosomes: novel effectors of human platelet lysate exercise. Eur Cell Mater. 2014;28:137–51. dialogue 151.

    CAS 
    PubMed 

    Google Scholar
     

  • 21.

    Montecalvo A, Larregina AT, Morelli AE. Strategies of study of dendritic cell-derived exosome-shuttle microRNA and its horizontal propagation between dendritic cells. Strategies Mol Biol. 2013;1024:19–40.

    CAS 
    PubMed 

    Google Scholar
     

  • 22.

    Guo SC, Tao SC, Yin WJ, Qi X, Yuan T, Zhang CQ. Exosomes derived from platelet-rich plasma promote the re-epithelization of power cutaneous wounds by way of activation of YAP in a diabetic rat mannequin. Theranostics. 2017;7(1):81–96.

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • 23.

    Tao SC, Yuan T, Rui BY, Zhu ZZ, Guo SC, Zhang CQ. Exosomes derived from human platelet-rich plasma forestall apoptosis induced by glucocorticoid-associated endoplasmic reticulum stress in rat osteonecrosis of the femoral head by way of the Akt/Dangerous/Bcl-2 sign pathway. Theranostics. 2017;7(3):733–50.

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • 24.

    Wu JP, Piao YX, Liu QY, Yang XY. Platelet-rich plasma-derived extracellular vesicles: a superior various in regenerative medication? Cell Prolif. 2021; 54(12):e13123. https://doi.org/10.1111/cpr.13123.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • 25.

    Ridder Ok, Keller S, Dams M, Rupp AK, Schlaudraff J, Turco DD, Starmann J, Macas J, Karpova D, Devraj Ok, Depboylu G, Landfried B, Arnold B, Plate KH, Höglinger G, Sültmann H, Altevogt P, Momma S. Extracellular vesicle-mediated switch of genetic info between the hematopoietic system and the mind in response to irritation. PLoS Biol. 2014;12(6):e1001874.

    PubMed 
    PubMed Central 

    Google Scholar
     

  • 26.

    Robbins PD, Morelli AE. Regulation of immune responses by extracellular vesicles. Nat Rev Immunol. 2014;14(3):195–208.

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • 27.

    Mol EA, Lei Z, Roefs MT, Bakker MH, Goumans MJ, Doevendans PA, Dankers PYW, Vader P, Sluijter JPG. Injectable supramolecular ureidopyrimidinone hydrogels present sustained launch of extracellular vesicle therapeutics. Adv Healthc Mater. 2019;8(20):e1900847.

    PubMed 

    Google Scholar
     

  • 28.

    Lv Ok, Li Q, Zhang L, Wang Y, Zhong Z, Zhao J, Lin X, Wang J, Zhu Ok, Xiao C, et al. Incorporation of small extracellular vesicles in sodium alginate hydrogel as a novel therapeutic technique for myocardial infarction. Theranostics. 2019;9(24):7403–16.

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • 29.

    Nikravesh N, Davies OG, Azoidis I, Moakes RJA, Marani L, Turner M, Kearney CJ, Eisenstein NM, Grover LM, Cox SC. Bodily structuring of injectable polymeric programs to controllably ship nanosized extracellular vesicles. Adv Healthc Mater. 2019;8(9):e1801604.

    PubMed 

    Google Scholar
     

  • 30.

    Pena B, Laughter M, Jett S, Rowland TJ, Taylor MRG, Mestroni L, Park D. Injectable hydrogels for cardiac tissue engineering. Macromol Biosci. 2018;18(6):e1800079.

    PubMed 
    PubMed Central 

    Google Scholar
     

  • 31.

    Moghimi SM, Hunter AC. Poloxamers and poloxamines in nanoparticle engineering and experimental medication. Traits Biotechnol. 2000;18(10):412–20.

    CAS 
    PubMed 

    Google Scholar
     

  • 32.

    Dumortier G, Grossiord JL, Agnely F, Chaumeil JC. A overview of poloxamer 407 pharmaceutical and pharmacological traits. Pharm Res. 2006;23(12):2709–28.

    CAS 
    PubMed 

    Google Scholar
     

  • 33.

    Liu D, Jiang T, Cai W, Chen J, Zhang H, Hietala S, Santos HA, Yin G, Fan J. An in situ gelling drug supply system for improved restoration after spinal wire harm. Adv Healthc Mater. 2016;5(12):1513–21.

    CAS 
    PubMed 

    Google Scholar
     

  • 34.

    Mulcahy LA, Pink RC, Carter DR. Routes and mechanisms of extracellular vesicle uptake. J Extracell Vesicles. 2014;3.

  • 35.

    Lee WY, Wang B. Cartilage restore by mesenchymal stem cells: scientific trial replace and views. J Orthop Translat. 2017;9:76–88.

    PubMed 
    PubMed Central 

    Google Scholar
     

  • 36.

    Spinal Wire Damage (SCI). 2016 Details and figures at a look. J Spinal Wire Med. 2016;39(4):493–4.


    Google Scholar
     

  • 37.

    Fernandez-Moure JS, Van Eps JL, Scherba JC, Yazdi IK, Robbins A, Cabrera F, Vatsaas C, Moreno M, Weiner BK, Tasciotti E. Platelet-rich plasma enhances mechanical energy of strattice in rat mannequin of ventral hernia restore. J Tissue Eng Regen Med. 2021;15(7):634–47.

    CAS 
    PubMed 

    Google Scholar
     

  • 38.

    Spinal wire harm details and figures at a look. J Spinal Wire Med. 2014;37(5):659–660.

  • 39.

    Li J, Chen H, Zhang D, Xie J, Zhou X. The position of stromal cell-derived issue 1 on cartilage improvement and illness. Osteoarthrit Cartil. 2021;29(3):313–22.

    CAS 

    Google Scholar
     

  • 40.

    Xiang X, Liu H, Wang L, Zhu B, Ma L, Du F, Li L, Qiu L. Ultrasound mixed with SDF-1alpha chemotactic microbubbles promotes stem cell homing in an osteoarthritis mannequin. J Cell Mol Med. 2020;24(18):10816–29.

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • 41.

    Fernandez-Pernas P, Rodriguez-Lesende I, de la Fuente A, Mateos J, Fuentes I, De Toro J, Blanco FJ, Arufe MC. CD105+-mesenchymal stem cells migrate into osteoarthritis joint: an animal mannequin. PLoS One. 2017;12(11):e0188072.

    PubMed 
    PubMed Central 

    Google Scholar
     

  • 42.

    Hasegawa S, Kitoh H, Ohkawara B, Mishima Ok, Matsushita M, Masuda A, Ishiguro N, Ohno Ok. Tranilast stimulates endochondral ossification by upregulating SOX9 and RUNX2 promoters. Biochem Biophys Res Commun. 2016;470(2):356–61.

    CAS 
    PubMed 

    Google Scholar
     

  • 43.

    Spinal Wire Damage (SCI). Details and figures at a look. J Spinal Wire Med. 2016;39(2):243–4.


    Google Scholar
     

  • 44.

    Li J, Zhao Z, Liu J, Huang N, Lengthy D, Wang J, Li X, Liu Y. MEK/ERK and p38 MAPK regulate chondrogenesis of rat bone marrow mesenchymal stem cells by means of delicate interplay with TGF-beta1/Smads pathway. Cell Prolif. 2010;43(4):333–43.

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • 45.

    Narcisi R, Cleary MA, Brama PA, Hoogduijn MJ, Tuysuz N, ten Berge D, van Osch GJ. Lengthy-term growth, enhanced chondrogenic potential, and suppression of endochondral ossification of grownup human MSCs by way of WNT signaling modulation. Stem Cell Rep. 2015;4(3):459–72.

    CAS 

    Google Scholar
     

  • 46.

    Correa D, Somoza RA, Lin P, Greenberg S, Rom E, Duesler L, Welter JF, Yayon A, Caplan AI. Sequential publicity to fibroblast development elements (FGF) 2, 9 and 18 enhances hMSC chondrogenic differentiation. Osteoarthrit Cartil. 2015;23(3):443–53.

    CAS 

    Google Scholar
     

  • 47.

    Szwedowski D, Szczepanek J, Paczesny L, Zabrzynski J, Gagat M, Mobasheri A, Jeka S. The impact of platelet-rich plasma on the intra-articular microenvironment in knee osteoarthritis. Int J Mol Sci. 2021;22(11).

  • 48.

    Kobayashi M, Squires GR, Mousa A, Tanzer M, Zukor DJ, Antoniou J, Feige U, Poole AR. Position of interleukin-1 and tumor necrosis issue alpha in matrix degradation of human osteoarthritic cartilage. Arthritis Rheum. 2005;52(1):128–35.

    CAS 
    PubMed 

    Google Scholar
     

  • 49.

    Andia I, Maffulli N. Platelet-rich plasma for managing ache and irritation in osteoarthritis. Nat Rev Rheumatol. 2013;9(12):721–30.

    CAS 
    PubMed 

    Google Scholar
     

  • 50.

    Umlauf D, Frank S, Pap T, Bertrand J. Cartilage biology, pathology, and restore. Cell Mol Life Sci. 2010;67(24):4197–211.

    CAS 
    PubMed 

    Google Scholar
     

  • 51.

    Troeberg L, Nagase H. Proteases concerned in cartilage matrix degradation in osteoarthritis. Biochim Biophys Acta. 2012;1824(1):133–45.

    CAS 
    PubMed 

    Google Scholar
     

  • 52.

    Lauer JC, Selig M, Hart ML, Kurz B, Rolauffs B. Articular chondrocyte phenotype regulation by means of the cytoskeleton and the signaling processes that originate from or converge on the cytoskeleton: in direction of a novel understanding of the intersection between actin dynamics and chondrogenic perform. Int J Mol Sci. 2021;22(6).

  • 53.

    Liu CF, Angelozzi M, Haseeb A, Lefebvre V. SOX9 is dispensable for the initiation of epigenetic transforming and the activation of marker genes on the onset of chondrogenesis. Improvement. 2018;145(14).

  • 54.

    Ferrao Blanco MN, Domenech Garcia H, Legeai-Mallet L, van Osch G. Tyrosine kinases regulate chondrocyte hypertrophy: promising drug targets for Osteoarthritis. Osteoarthrit Cartil. 2021;29(10):1389–98.

    CAS 

    Google Scholar
     

  • 55.

    Ji Q, Zheng Y, Zhang G, Hu Y, Fan X, Hou Y, Wen L, Li L, Xu Y, Wang Y, Tang F. Single-cell RNA-seq evaluation reveals the development of human osteoarthritis. Ann Rheum Dis. 2019;78(1):100–10.

    CAS 
    PubMed 

    Google Scholar
     

  • 56.

    Chou CH, Jain V, Gibson J, Attarian DE, Haraden CA, Yohn CB, Laberge RM, Gregory S, Kraus VB. Synovial cell cross-talk with cartilage performs a serious position within the pathogenesis of osteoarthritis. Sci Rep. 2020;10(1):10868.

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • 57.

    Yan D, Chen D, Cool SM, van Wijnen AJ, Mikecz Ok, Murphy G, Im HJ. Fibroblast development issue receptor 1 is principally liable for fibroblast development issue 2-induced catabolic actions in human articular chondrocytes. Arthritis Res Ther. 2011;13(4):R130.

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • 58.

    Mevel E, Shutter JA, Ding X, Mattingly BT, Williams JN, Li Y, Huls A, Kambrath AV, Trippel SB, Wagner D, et al. Systemic inhibition or world deletion of CaMKK2 protects towards post-traumatic osteoarthritis. Osteoarthrit Cartil. 2022; 30(1):124-36.

  • 59.

    Adan N, Guzman-Morales J, Ledesma-Colunga MG, Perales-Canales SI, Quintanar-Stephano A, Lopez-Barrera F, Mendez I, Moreno-Carranza B, Triebel J, Binart N, et al. Prolactin promotes cartilage survival and attenuates irritation in inflammatory arthritis. J Clin Make investments. 2013;123(9):3902–13.

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • 60.

    Minashima T, Zhang Y, Lee Y, Kirsch T. Lithium protects towards cartilage degradation in osteoarthritis. Arthritis Rheumatol. 2014;66(5):1228–36.

    CAS 
    PubMed 

    Google Scholar
     

  • 61.

    Kihara S, Hayashi S, Hashimoto S, Kanzaki N, Takayama Ok, Matsumoto T, Chinzei N, Iwasa Ok, Haneda M, Takeuchi Ok, et al. Cyclin-dependent kinase inhibitor-1-deficient mice are inclined to osteoarthritis related to enhanced irritation. J Bone Miner Res. 2017;32(5):991–1001.

    CAS 
    PubMed 

    Google Scholar
     

  • 62.

    Yao ZZ, Hu AX, Liu XS. DUSP19 regulates IL-1beta-induced apoptosis and MMPs expression in rat chondrocytes by means of JAK2/STAT3 signaling pathway. Biomed Pharmacother. 2017;96:1209–15.

    CAS 
    PubMed 

    Google Scholar
     

  • 63.

    Liang T, Chen T, Qiu J, Gao W, Qiu X, Zhu Y, Wang X, Chen Y, Zhou H, Deng Z, et al. Inhibition of nuclear receptor RORalpha attenuates cartilage harm in osteoarthritis by modulating IL-6/STAT3 pathway. Cell Dying Dis. 2021;12(10):886.

    PubMed 
    PubMed Central 

    Google Scholar
     

  • 64.

    Zhan J, Yan Z, Kong X, Liu J, Lin Z, Qi W, Wu Y, Lin J, Pan X, Xue X. Lycopene inhibits IL-1beta-induced irritation in mouse chondrocytes and mediates murine osteoarthritis. J Cell Mol Med. 2021;25(7):3573–84.

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • 65.

    Lu W, Ding Z, Liu F, Shan W, Cheng C, Xu J, He W, Huang W, Ma J, Yin Z. Dopamine delays articular cartilage degradation in osteoarthritis by unfavourable regulation of the NF-kappaB and JAK2/STAT3 signaling pathways. Biomed Pharmacother. 2019;119:109419.

    CAS 
    PubMed 

    Google Scholar
     

  • 66.

    Younas A, Gu H, Zhao Y, Zhang N. Novel approaches of the nanotechnology-based drug supply programs for knee joint accidents: a overview. Int J Pharm. 2021;608:121051.

    CAS 
    PubMed 

    Google Scholar
     

  • 67.

    Tao SC, Guo SC, Zhang CQ. Platelet-derived extracellular vesicles: an rising therapeutic method. Int J Biol Sci. 2017;13(7):828–34.

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • 68.

    Melki I, Tessandier N, Zufferey A, Boilard E. Platelet microvesicles in well being and illness. Platelets. 2017;28(3):214–21.

    CAS 
    PubMed 

    Google Scholar
     

  • 69.

    Johnson J, Wu YW, Blyth C, Lichtfuss G, Goubran H, Burnouf T. Potential therapeutic purposes of platelet extracellular vesicles. Traits Biotechnol. 2021;39(6):598–612.

    CAS 
    PubMed 

    Google Scholar
     

  • 70.

    Boilard E. Extracellular vesicles and their content material in bioactive lipid mediators: greater than a sack of microRNA. J Lipid Res. 2018;59(11):2037–46.

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • 71.

    Montaseri A, Busch F, Mobasheri A, Buhrmann C, Aldinger C, Rad JS, Shakibaei M. IGF-1 and PDGF-bb suppress IL-1beta-induced cartilage degradation by means of down-regulation of NF-kappaB signaling: involvement of Src/PI-3 Ok/AKT pathway. PLoS One. 2011;6(12):e28663.

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • 72.

    van Buul GM, Koevoet WL, Kops N, Bos PK, Verhaar JA, Weinans H, Bernsen MR, van Osch GJ. Platelet-rich plasma releasate inhibits inflammatory processes in osteoarthritic chondrocytes. Am J Sports activities Med. 2011;39(11):2362–70.

    PubMed 

    Google Scholar
     

  • 73.

    Huang W, Begum R, Barber T, Ibba V, Tee NC, Hussain M, Arastoo M, Yang Q, Robson LG, Lesage S, et al. Regenerative potential of silk conduits in restore of peripheral nerve harm in grownup rats. Biomaterials. 2012;33(1):59–71.

    PubMed 

    Google Scholar
     

  • 74.

    Ferrao Blanco MN, Bastiaansen-Jenniskens YM, Chambers MG, Pitsillides AA, Narcisi R, van Osch GJVM. Impact of inflammatory signaling on human articular chondrocyte hypertrophy: potential involvement of tissue restore macrophages. Cartilage. 2021;13(2_suppl):168S-74S. https://doi.org/10.1177/19476035211021907.

    CAS 
    Article 
    PubMed 

    Google Scholar
     

  • 75.

    Kim HY, Wang J, Chung Ok, Chung JM. A surgical ankle sprain ache mannequin within the rat: results of morphine and indomethacin. Neurosci Lett. 2008;442(2):161–4.

    CAS 
    PubMed 

    Google Scholar
     

  • 76.

    Liu P, Chen Ok, Wang S, Hua C, Zhang H, Yu J. A mouse mannequin of ankle-subtalar joint advanced instability induced post-traumatic osteoarthritis. J Orthop Surg Res. 2021;16(1):541.

    PubMed 
    PubMed Central 

    Google Scholar
     

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